Protein-coding gene in the species Homo sapiens
EPS15 Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1C07 , 1EH2 , 1F8H , 1FF1 , 2IV9 , 2JXC , 4RH5 , 4RH9 , 4RHG , 4S0G , 5JP2
Identifiers Aliases EPS15 , AF-1P, AF1P, MLLT5, epidermal growth factor receptor pathway substrate 15External IDs OMIM : 600051 ; MGI : 104583 ; HomoloGene : 128359 ; GeneCards : EPS15 ; OMA :EPS15 - orthologs Wikidata
Epidermal growth factor receptor substrate 15 is a protein that in humans is encoded by the EPS15 gene .[ 5]
Function
This gene encodes a protein that is part of the EGFR pathway. The protein is present at clathrin-coated pits and is involved in receptor-mediated endocytosis of EGF. Notably, this gene is rearranged with the HRX/ALL/MLL gene in acute myelogeneous leukemias . Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.[ 6]
Interactions
EPS15 has been shown to interact with:
References
^ a b c GRCh38: Ensembl release 89: ENSG00000085832 – Ensembl , May 2017
^ a b c GRCm38: Ensembl release 89: ENSMUSG00000028552 – Ensembl , May 2017
^ "Human PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .
^ "Mouse PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .
^ Wong WT, Kraus MH, Carlomagno F, Zelano A, Druck T, Croce CM, Huebner K, Di Fiore PP (Jun 1994). "The human eps15 gene, encoding a tyrosine kinase substrate, is conserved in evolution and maps to 1p31-p32". Oncogene . 9 (6): 1591–7. PMID 8183552 .
^ "Entrez Gene: EPS15 epidermal growth factor receptor pathway substrate 15" .
^ Schumacher C, Knudsen BS, Ohuchi T, Di Fiore PP, Glassman RH, Hanafusa H (June 1995). "The SH3 domain of Crk binds specifically to a conserved proline-rich motif in Eps15 and Eps15R" . J. Biol. Chem . 270 (25): 15341–7. doi :10.1074/jbc.270.25.15341 . PMID 7797522 .
^ Chen H, Fre S, Slepnev VI, Capua MR, Takei K, Butler MH, Di Fiore PP, De Camilli P (August 1998). "Epsin is an EH-domain-binding protein implicated in clathrin-mediated endocytosis". Nature . 394 (6695): 793–7. Bibcode :1998Natur.394..793C . doi :10.1038/29555 . PMID 9723620 . S2CID 4430975 .
^ Bean AJ, Davanger S, Chou MF, Gerhardt B, Tsujimoto S, Chang Y (May 2000). "Hrs-2 regulates receptor-mediated endocytosis via interactions with Eps15" . J. Biol. Chem . 275 (20): 15271–8. doi :10.1074/jbc.275.20.15271 . PMID 10809762 .
^ Bache KG, Raiborg C, Mehlum A, Stenmark H (April 2003). "STAM and Hrs are subunits of a multivalent ubiquitin-binding complex on early endosomes" . J. Biol. Chem . 278 (14): 12513–21. doi :10.1074/jbc.M210843200 . PMID 12551915 .
^ Doria M, Salcini AE, Colombo E, Parslow TG, Pelicci PG, Di Fiore PP (December 1999). "The eps15 homology (EH) domain-based interaction between eps15 and hrb connects the molecular machinery of endocytosis to that of nucleocytosolic transport" . J. Cell Biol . 147 (7): 1379–84. doi :10.1083/jcb.147.7.1379 . PMC 2174238 . PMID 10613896 .
^ Nakashima S, Morinaka K, Koyama S, Ikeda M, Kishida M, Okawa K, Iwamatsu A, Kishida S, Kikuchi A (July 1999). "Small G protein Ral and its downstream molecules regulate endocytosis of EGF and insulin receptors" . EMBO J . 18 (13): 3629–42. doi :10.1093/emboj/18.13.3629 . PMC 1171441 . PMID 10393179 .
Further reading
Schumacher C, Knudsen BS, Ohuchi T, Di Fiore PP, Glassman RH, Hanafusa H (1995). "The SH3 domain of Crk binds specifically to a conserved proline-rich motif in Eps15 and Eps15R" . J. Biol. Chem . 270 (25): 15341–7. doi :10.1074/jbc.270.25.15341 . PMID 7797522 .
Bernard OA, Mauchauffe M, Mecucci C, Van den Berghe H, Berger R (1994). "A novel gene, AF-1p, fused to HRX in t(1;11)(p32;q23), is not related to AF-4, AF-9 nor ENL". Oncogene . 9 (4): 1039–45. PMID 8134107 .
Andersson B, Wentland MA, Ricafrente JY, Liu W, Gibbs RA (1996). "A "double adaptor" method for improved shotgun library construction". Anal. Biochem . 236 (1): 107–13. doi :10.1006/abio.1996.0138 . PMID 8619474 .
Benmerah A, Bégue B, Dautry-Varsat A, Cerf-Bensussan N (1996). "The ear of alpha-adaptin interacts with the COOH-terminal domain of the Eps 15 protein" . J. Biol. Chem . 271 (20): 12111–6. doi :10.1074/jbc.271.20.12111 . PMID 8662627 .
Matsuda M, Ota S, Tanimura R, Nakamura H, Matuoka K, Takenawa T, Nagashima K, Kurata T (1996). "Interaction between the amino-terminal SH3 domain of CRK and its natural target proteins" . J. Biol. Chem . 271 (24): 14468–72. doi :10.1074/jbc.271.24.14468 . PMID 8662907 .
van Delft S, Schumacher C, Hage W, Verkleij AJ, van Bergen en Henegouwen PM (1997). "Association and colocalization of Eps15 with adaptor protein-2 and clathrin" . J. Cell Biol . 136 (4): 811–21. doi :10.1083/jcb.136.4.811 . PMC 2132490 . PMID 9049247 .
Yu W, Andersson B, Worley KC, Muzny DM, Ding Y, Liu W, Ricafrente JY, Wentland MA, Lennon G, Gibbs RA (1997). "Large-scale concatenation cDNA sequencing" . Genome Res . 7 (4): 353–8. doi :10.1101/gr.7.4.353 . PMC 139146 . PMID 9110174 .
Tebar F, Confalonieri S, Carter RE, Di Fiore PP, Sorkin A (1997). "Eps15 is constitutively oligomerized due to homophilic interaction of its coiled-coil region" . J. Biol. Chem . 272 (24): 15413–8. doi :10.1074/jbc.272.24.15413 . PMID 9182572 .
Salcini AE, Confalonieri S, Doria M, Santolini E, Tassi E, Minenkova O, Cesareni G, Pelicci PG, Di Fiore PP (1997). "Binding specificity and in vivo targets of the EH domain, a novel protein-protein interaction module" . Genes Dev . 11 (17): 2239–49. doi :10.1101/gad.11.17.2239 . PMC 275390 . PMID 9303539 .
Cupers P, ter Haar E, Boll W, Kirchhausen T (1998). "Parallel dimers and anti-parallel tetramers formed by epidermal growth factor receptor pathway substrate clone 15" . J. Biol. Chem . 272 (52): 33430–4. doi :10.1074/jbc.272.52.33430 . PMID 9407139 .
Haffner C, Takei K, Chen H, Ringstad N, Hudson A, Butler MH, Salcini AE, Di Fiore PP, De Camilli P (1998). "Synaptojanin 1: localization on coated endocytic intermediates in nerve terminals and interaction of its 170 kDa isoform with Eps15". FEBS Lett . 419 (2–3): 175–80. doi :10.1016/S0014-5793(97)01451-8 . PMID 9428629 . S2CID 45186831 .
Benmerah A, Lamaze C, Bègue B, Schmid SL, Dautry-Varsat A, Cerf-Bensussan N (1998). "AP-2/Eps15 interaction is required for receptor-mediated endocytosis" . J. Cell Biol . 140 (5): 1055–62. doi :10.1083/jcb.140.5.1055 . PMC 2132690 . PMID 9490719 .
de Beer T, Carter RE, Lobel-Rice KE, Sorkin A, Overduin M (1998). "Structure and Asn-Pro-Phe binding pocket of the Eps15 homology domain". Science . 281 (5381): 1357–60. Bibcode :1998Sci...281.1357D . doi :10.1126/science.281.5381.1357 . PMID 9721102 .
Chen H, Fre S, Slepnev VI, Capua MR, Takei K, Butler MH, Di Fiore PP, De Camilli P (1998). "Epsin is an EH-domain-binding protein implicated in clathrin-mediated endocytosis". Nature . 394 (6695): 793–7. Bibcode :1998Natur.394..793C . doi :10.1038/29555 . PMID 9723620 . S2CID 4430975 .
Lewin DA, Sheff D, Ooi CE, Whitney JA, Yamamoto E, Chicione LM, Webster P, Bonifacino JS, Mellman I (1998). "Cloning, expression, and localization of a novel gamma-adaptin-like molecule". FEBS Lett . 435 (2–3): 263–8. doi :10.1016/S0014-5793(98)01083-7 . PMID 9762922 . S2CID 83518205 .
Sengar AS, Wang W, Bishay J, Cohen S, Egan SE (1999). "The EH and SH3 domain Ese proteins regulate endocytosis by linking to dynamin and Eps15" . EMBO J . 18 (5): 1159–71. doi :10.1093/emboj/18.5.1159 . PMC 1171207 . PMID 10064583 .
Poupon V, Bègue B, Gagnon J, Dautry-Varsat A, Cerf-Bensussan N, Benmerah A (1999). "Molecular cloning and characterization of MT-ACT48, a novel mitochondrial acyl-CoA thioesterase" . J. Biol. Chem . 274 (27): 19188–94. doi :10.1074/jbc.274.27.19188 . PMID 10383425 .
Nakashima S, Morinaka K, Koyama S, Ikeda M, Kishida M, Okawa K, Iwamatsu A, Kishida S, Kikuchi A (1999). "Small G protein Ral and its downstream molecules regulate endocytosis of EGF and insulin receptors" . EMBO J . 18 (13): 3629–42. doi :10.1093/emboj/18.13.3629 . PMC 1171441 . PMID 10393179 .
Stephens DJ, Banting G (1999). "Direct interaction of the trans-Golgi network membrane protein, TGN38, with the F-actin binding protein, neurabin" . J. Biol. Chem . 274 (42): 30080–6. doi :10.1074/jbc.274.42.30080 . PMID 10514494 .
PDB gallery
1c07 : STRUCTURE OF THE THIRD EPS15 HOMOLOGY DOMAIN OF HUMAN EPS15
1eh2 : STRUCTURE OF THE SECOND EPS15 HOMOLOGY DOMAIN OF HUMAN EPS15, NMR, 20 STRUCTURES
1f8h : STRUCTURE OF THE SECOND EPS15 HOMOLOGY DOMAIN OF HUMAN EPS15 IN COMPLEX WITH PTGSSSTNPFR
1ff1 : STRUCTURE OF THE SECOND EPS15 HOMOLOGY DOMAIN OF HUMAN EPS15 IN COMPLEX WITH PTGSSSTNPFL
1qjt : SOLUTION STRUCTURE OF THE APO EH1 DOMAIN OF MOUSE EPIDERMAL GROWTH FACTOR RECEPTOR SUBSTRATE 15, EPS15